58型重组人乳头瘤病毒病毒样颗粒及其制备方法

Glu Ile Gly Arg Gly Gln Pro Leu Gly Val Gly Leu Ser Gly His Pro

340 345 350

Phe Tyr Asn Lys Leu Asp Asp Thr Glu Ser Ser His Ala Ala Thr Ser

355 360 365

Asn Val Ser Glu Asp Val Arg Asp Asn Val Ser Val Asp Tyr Lys Gln

370 375 380

Thr Gln Leu Cys Ile Leu Gly Cys Ala Pro Ala Ile Gly Glu His Trp

385 390 395 400

Ala Lys Gly Thr Ala Cys Lys Ser Arg Pro Leu Ser Gln Gly Asp Cys

405 410 415

Pro Pro Leu Glu Leu Lys Asn Thr Val Leu Glu Asp Gly Asp Met Val

420 425 430

Asp Thr Gly Tyr Gly Ala Met Asp Phe Ser Thr Leu Gln Asp Thr Lys

435 440 445

Cys Glu Val Pro Leu Asp Ile Cys Gln Ser Ile Cys Lys Tyr Pro Asp

450 455 460

Tyr Leu Gln Met Ser Ala Asp Pro Tyr Gly Asp Ser Met Phe Phe Cys

465 470 475 480

Leu Arg Arg Glu Gln Leu Phe Ala Arg His Phe Trp Asn Arg Ala Gly

485 490 495

Thr Met Gly Asp Thr Val Pro Gln Ser Leu Tyr Ile Lys Gly Thr Gly

500 505 510

Met Arg Ala Ser Pro Gly Ser Cys Val Tyr Ser Pro Ser Pro Ser Gly

515 520 525

Ser Ile Val Thr Ser Asp Ser Gln Leu Phe Asn Lys Pro Tyr Trp Leu

530 535 540

His Lys Ala Gln Gly His Asn Asn Gly Val Cys Trp His Asn Gln Leu

545 550 555 560

Phe Val Thr Val Val Asp Thr Thr Arg Ser Thr Asn Leu Thr Ile Cys

565 570 575

Ala Ser Thr Gln Ser Pro Val Pro Gly Gln Tyr Asp Ala Thr Lys Phe

580 585 590

Lys Gln Tyr Ser Arg His Val Glu Glu Tyr Asp Leu Gln Phe Ile Phe

595 600 605

Gln Leu Cys Thr Ile Thr Leu Thr Ala Asp Val Met Ser Tyr Ile His

610 615 620

Ser Met Asn Ser Ser Ile Leu Glu Asp Trp Asn Phe Gly Val Pro Pro

625 630 635 640

Pro Pro Thr Thr Ser Leu Val Asp Thr Tyr Arg Phe Val Gln Ser Val

645 650 655

Ala Ile Thr Cys Gln Lys Asp Ala Ala Pro Ala Glu Asn Lys Asp Pro

660 665 670

Tyr Asp Lys Leu Lys Phe Trp Asn Val Asp Leu Lys Glu Lys Phe Ser

675 680 685

Leu Asp Leu Asp Gln Tyr Pro Leu Gly Arg Lys Phe Leu Val Gln Ala

690 695 700

Gly Leu Arg Arg Lys Pro Thr Ile Gly Pro Arg Lys Arg Ser Ala Pro

705 710 715 720

Ser Ala Thr Thr Ser Ser Lys Pro Ala Lys Arg Val Arg Val Arg Ala

725 730 735

Arg Lys

<210>9

<211>832

<212>PRT

<213>人工序列

<400>9

Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro

1 5 1015

Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu

202530

Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu

354045

Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys

50 5560

Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn

65707580

Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu

859095

Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser

100 105 110

Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu

115 120 125

Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn

130 135 140

Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp

145 150 155 160

Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu

165 170 175

Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr

180 185 190

Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala

195 200 205

Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Leu Glu Val Leu

210 215 220

Phe Gln Gly Pro Ser Asp Gln Glu Ala Lys Pro Ser Thr Glu Asp Leu

225 230 235 240

Gly Asp Lys Lys Glu Gly Glu Tyr Ile Lys Leu Lys Val Ile Gly Gln

245 250 255

Asp Ser Ser Glu Ile His Phe Lys Val Lys Met Thr Thr His Leu Lys

260 265 270

Lys Leu Lys Glu Ser Tyr Cys Gln Arg Gln Gly Val Pro Met Asn Ser

275 280 285

Leu Arg Phe Leu Phe Glu Gly Gln Arg Ile Ala Asp Asn His Thr Pro

290 295 300

Lys Glu Leu Gly Met Glu Glu Glu Asp Val Ile Glu Val Tyr Gln Glu

305 310 315 320

Gln Thr Gly Gly Ser Met Ala Leu Trp Arg Pro Ser Asp Asn Thr Val

325 330 335

Tyr Leu Pro Pro Pro Ser Val Ala Arg Val Val Asn Thr Asp Asp Tyr

340 345 350

Val Thr Arg Thr Ser Ile Phe Tyr His Ala Gly Ser Ser Arg Leu Leu

355 360 365

Thr Val Gly Asn Pro Tyr Phe Arg Val Pro Ala Gly Gly Gly Asn Lys

370 375 380

Gln Asp Ile Pro Lys Val Ser Ala Tyr Gln Tyr Arg Val Phe Arg Val

385 390 395 400

Gln Leu Pro Asp Pro Asn Lys Phe Gly Leu Pro Asp Asn Ser Ile Tyr

405 410 415

Asn Pro Glu Thr Gln Arg Leu Val Trp Ala Cys Ala Gly Val Glu Ile

420 425 430

Gly Arg Gly Gln Pro Leu Gly Val Gly Leu Ser Gly His Pro Phe Tyr

435 440 445

Asn Lys Leu Asp Asp Thr Glu Ser Ser His Ala Ala Thr Ser Asn Val

450 455 460

Ser Glu Asp Val Arg Asp Asn Val Ser Val Asp Tyr Lys Gln Thr Gln

465 470 475 480

Leu Cys Ile Leu Gly Cys Ala Pro Ala Ile Gly Glu His Trp Ala Lys

485 490 495

Gly Thr Ala Cys Lys Ser Arg Pro Leu Ser Gln Gly Asp Cys Pro Pro

500 505 510

Leu Glu Leu Lys Asn Thr Val Leu Glu Asp Gly Asp Met Val Asp Thr

515 520 525

Gly Tyr Gly Ala Met Asp Phe Ser Thr Leu Gln Asp Thr Lys Cys Glu

530 535 540

Val Pro Leu Asp Ile Cys Gln Ser Ile Cys Lys Tyr Pro Asp Tyr Leu

545 550 555 560

Gln Met Ser Ala Asp Pro Tyr Gly Asp Ser Met Phe Phe Cys Leu Arg

565 570 575

Arg Glu Gln Leu Phe Ala Arg His Phe Trp Asn Arg Ala Gly Thr Met

580 585 590

Gly Asp Thr Val Pro Gln Ser Leu Tyr Ile Lys Gly Thr Gly Met Arg

595 600 605

Ala Ser Pro Gly Ser Cys Val Tyr Ser Pro Ser Pro Ser Gly Ser Ile

610 615 620

Val Thr Ser Asp Ser Gln Leu Phe Asn Lys Pro Tyr Trp Leu His Lys

625 630 635 640

Ala Gln Gly His Asn Asn Gly Val Cys Trp His Asn Gln Leu Phe Val

645 650 655

Thr Val Val Asp Thr Thr Arg Ser Thr Asn Leu Thr Ile Cys Ala Ser

660 665 670

Thr Gln Ser Pro Val Pro Gly Gln Tyr Asp Ala Thr Lys Phe Lys Gln

675 680 685

Tyr Ser Arg His Val Glu Glu Tyr Asp Leu Gln Phe Ile Phe Gln Leu

690 695 700

Cys Thr Ile Thr Leu Thr Ala Asp Val Met Ser Tyr Ile His Ser Met

705 710 715 720

Asn Ser Ser Ile Leu Glu Asp Trp Asn Phe Gly Val Pro Pro Pro Pro

725 730 735

Thr Thr Ser Leu Val Asp Thr Tyr Arg Phe Val Gln Ser Val Ala Ile

740 745 750

Thr Cys Gln Lys Asp Ala Ala Pro Ala Glu Asn Lys Asp Pro Tyr Asp

755 760 765

Lys Leu Lys Phe Trp Asn Val Asp Leu Lys Glu Lys Phe Ser Leu Asp

770 775 780

Leu Asp Gln Tyr Pro Leu Gly Arg Lys Phe Leu Val Gln Ala Gly Leu

785 790 795 800

Arg Arg Lys Pro Thr Ile Gly Pro Arg Lys Arg Ser Ala Pro Ser Ala

805 810 815

Thr Thr Ser Ser Lys Pro Ala Lys Arg Val Arg Val Arg Ala Arg Lys

820 825 830

<210>10

<211>898

<212>PRT

<213>人工序列

<400>10

Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys

1 5 1015

Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr

202530

Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe

354045

Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala

505560

His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile

65707580

Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp

859095

Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu

100 105 110

Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys

115 120 125

Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly

130 135 140

Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro

145 150 155 160

Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys

165 170 175

Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly

180 185 190

Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp

195 200 205

Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala

210 215 220

Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys

225 230 235 240

Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser

245 250 255

Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro

260 265 270

Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp

275 280 285

Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala

290 295 300

Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala

305 310 315 320

Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln

325 330 335

Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala

340 345 350

Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn

355 360 365

Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile

370 375 380

Glu Gly Arg Ile Ser Glu Phe Met Ala Leu Trp Arg Pro Ser Asp Asn

385 390 395 400

Thr Val Tyr Leu Pro Pro Pro Ser Val Ala Arg Val Val Asn Thr Asp

405 410 415

Asp Tyr Val Thr Arg Thr Ser Ile Phe Tyr His Ala Gly Ser Ser Arg

420 425 430

Leu Leu Thr Val Gly Asn Pro Tyr Phe Arg Val Pro Ala Gly Gly Gly

435 440 445

Asn Lys Gln Asp Ile Pro Lys Val Ser Ala Tyr Gln Tyr Arg Val Phe

450 455 460

Arg Val Gln Leu Pro Asp Pro Asn Lys Phe Gly Leu Pro Asp Asn Ser

465 470 475 480

Ile Tyr Asn Pro Glu Thr Gln Arg Leu Val Trp Ala Cys Ala Gly Val

485 490 495

Glu Ile Gly Arg Gly Gln Pro Leu Gly Val Gly Leu Ser Gly His Pro

500 505 510

Phe Tyr Asn Lys Leu Asp Asp Thr Glu Ser Ser His Ala Ala Thr Ser

515 520 525

Asn Val Ser Glu Asp Val Arg Asp Asn Val Ser Val Asp Tyr Lys Gln

530 535 540

Thr Gln Leu Cys Ile Leu Gly Cys Ala Pro Ala Ile Gly Glu His Trp

545 550 555 560

Ala Lys Gly Thr Ala Cys Lys Ser Arg Pro Leu Ser Gln Gly Asp Cys

565 570 575

Pro Pro Leu Glu Leu Lys Asn Thr Val Leu Glu Asp Gly Asp Met Val

580 585 590

Asp Thr Gly Tyr Gly Ala Met Asp Phe Ser Thr Leu Gln Asp Thr Lys

595 600 605

Cys Glu Val Pro Leu Asp Ile Cys Gln Ser Ile Cys Lys Tyr Pro Asp

610 615 620

Tyr Leu Gln Met Ser Ala Asp Pro Tyr Gly Asp Ser Met Phe Phe Cys

625 630 635 640

Leu Arg Arg Glu Gln Leu Phe Ala Arg His Phe Trp Asn Arg Ala Gly

645 650 655

Thr Met Gly Asp Thr Val Pro Gln Ser Leu Tyr Ile Lys Gly Thr Gly

660 665 670

Met Arg Ala Ser Pro Gly Ser Cys Val Tyr Ser Pro Ser Pro Ser Gly

675 680 685

Ser Ile Val Thr Ser Asp Ser Gln Leu Phe Asn Lys Pro Tyr Trp Leu

690 695 700

His Lys Ala Gln Gly His Asn Asn Gly Val Cys Trp His Asn Gln Leu

705 710 715 720

Phe Val Thr Val Val Asp Thr Thr Arg Ser Thr Asn Leu Thr Ile Cys

725 730 735

Ala Ser Thr Gln Ser Pro Val Pro Gly Gln Tyr Asp Ala Thr Lys Phe

740 745 750

Lys Gln Tyr Ser Arg His Val Glu Glu Tyr Asp Leu Gln Phe Ile Phe

755 760 765

Gln Leu Cys Thr Ile Thr Leu Thr Ala Asp Val Met Ser Tyr Ile His

770 775 780

Ser Met Asn Ser Ser Ile Leu Glu Asp Trp Asn Phe Gly Val Pro Pro

785 790 795 800

Pro Pro Thr Thr Ser Leu Val Asp Thr Tyr Arg Phe Val Gln Ser Val

805 810 815

Ala Ile Thr Cys Gln Lys Asp Ala Ala Pro Ala Glu Asn Lys Asp Pro

820 825 830

Tyr Asp Lys Leu Lys Phe Trp Asn Val Asp Leu Lys Glu Lys Phe Ser

835 840 845

Leu Asp Leu Asp Gln Tyr Pro Leu Gly Arg Lys Phe Leu Val Gln Ala

850 855 860

Gly Leu Arg Arg Lys Pro Thr Ile Gly Pro Arg Lys Arg Ser Ala Pro

865 870 875 880

Ser Ala Thr Thr Ser Ser Lys Pro Ala Lys Arg Val Arg Val Arg Ala

885 890 895

Arg Lys

<210>11

<211>2094

<212>DNA

<213>人工序列

<400>11

atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt 60

ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa120

tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat180

ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac240

atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg300

gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt360

gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa420

acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat480

gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa540

aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca600

tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat660

ctggaagttc tgttccaggg gccctctgaa gctaccgttt acctgccgcc ggttccggtt720

tctaaagttg tttctaccga cgaatacgtt tctcgtacct ctatctacta ctacgctggt780

tcttctcgtc tgctggctgt tggtaacccg tacttctcta tcaaatctcc gaacaacaac840

aaaaaagttc tggttccgaa agtttctggt ctgcagtacc gtgttttccg tgttcgtctg900

ccggacccga acaaattcgg tttcccggac acctctttct acaacccgga cacccagcgt960

ctggtttggg cttgcgttgg tctggaaatc ggtcgtggtc agccgctggg tgttggtgtt 1020

tctggtcacc cgtacttcaa caaattcgac gacaccgaaa cctctaaccg ttacccggct 1080

cagccgggtt ctgacaaccg tgaatgcctg tctatggact acaaacagac ccagctgtgc 1140

ctgatcggtt gcaaaccgcc gaccggtgaa cactggggta aaggtgttgc ttgcaacaac 1200

aacgctgctg ctaccgactg cccgccgctg gaactgttca actctatcat cgaagacggt 1260

gacatggttg acaccggttt cggttgcatg gacttcggta ccctgcaggc taacaaatct 1320

gacgttccga tcgacatctg caactctacc tgcaaatacc cggactacct gaaaatggct 1380

tctgaaccgt acggtgactc tctgttcttc ttcctgcgtc gtgaacagat gttcgttcgt 1440

cacttcttca accgtgctgg taaactgggt gaagctgttc cggacgacct gtacatcaaa 1500

ggttctggta acaccgctgt tatccagtct tctgctttct tcccgacccc gtctggttct 1560

atggttacct ctgaatctca gctgttcaac aaaccgtact ggctgcagcg tgctcagggt 1620

cacaacaacg gtatctgctg gggtaaccag ctgttcgtta ccgttgttga caccacccgt 1680

tctaccaaca tgaccctgtg caccgaagtt accaaagaag gtacctacaa aaacgacaac 1740

ttcaaagaat acgttcgtca cgttgaagaa tacgacctgc agttcgtttt ccagctgtgc 1800

aaaatcaccc tgaccgctga aatcatgacc tacatccaca ccatggactc taacatcctg 1860

gaagactggc agttcggtct gaccccgccg ccgtctgctt ctctgcagga cacctaccgt 1920

ttcgttacct ctcaggctat cacctgccag aaaaccgctc cgccgaaaga aaaagaagac 1980

ccgctgaaca aatacacctt ctgggaagtt aacctgaaag aaaaattctc tgctgacctg 2040

gaccagttcc cgctgggtcg taaattcctg ctgcagtctg gtctgaaagc ttag 2094

<210>12

<211>2451

<212>DNA

<213>人工序列

<400>12

atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt 60

ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa120

tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat180

ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac240

atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg300

gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt360

gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa420

acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat480

gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa540

aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca600

tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat660

ctggaagttc tgttccaggg gccctctgac caggaagcta aaccgtctac cgaagacctg720

ggtgacaaaa aagaaggtga atacatcaaa ctgaaagtta tcggtcagga ctcttctgaa780

atccacttca aagttaaaat gaccacccac ctgaaaaaac tgaaagaatc ttactgccag840

cgtcagggtg ttccgatgaa ctctctgcgt ttcctgttcg aaggtcagcg tatcgctgac900

aaccacaccc cgaaagaact gggtatggaa gaagaagacg ttatcgaagt ttaccaggaa960

cagaccggtg gatcctctga agctaccgtt tacctgccgc cggttccggt ttctaaagtt 1020

gtttctaccg acgaatacgt ttctcgtacc tctatctact actacgctgg ttcttctcgt 1080

ctgctggctg ttggtaaccc gtacttctct atcaaatctc cgaacaacaa caaaaaagtt 1140

ctggttccga aagtttctgg tctgcagtac cgtgttttcc gtgttcgtct gccggacccg 1200

aacaaattcg gtttcccgga cacctctttc tacaacccgg acacccagcg tctggtttgg 1260

gcttgcgttg gtctggaaat cggtcgtggt cagccgctgg gtgttggtgt ttctggtcac 1320

ccgtacttca acaaattcga cgacaccgaa acctctaacc gttacccggc tcagccgggt 1380

tctgacaacc gtgaatgcct gtctatggac tacaaacaga cccagctgtg cctgatcggt 1440

tgcaaaccgc cgaccggtga acactggggt aaaggtgttg cttgcaacaa caacgctgct 1500

gctaccgact gcccgccgct ggaactgttc aactctatca tcgaagacgg tgacatggtt 1560

gacaccggtt tcggttgcat ggacttcggt accctgcagg ctaacaaatc tgacgttccg 1620

atcgacatct gcaactctac ctgcaaatac ccggactacc tgaaaatggc ttctgaaccg 1680

tacggtgact ctctgttctt cttcctgcgt cgtgaacaga tgttcgttcg tcacttcttc 1740

aaccgtgctg gtaaactggg tgaagctgtt ccggacgacc tgtacatcaa aggttctggt 1800

aacaccgctg ttatccagtc ttctgctttc ttcccgaccc cgtctggttc tatggttacc 1860

tctgaatctc agctgttcaa caaaccgtac tggctgcagc gtgctcaggg tcacaacaac 1920

ggtatctgct ggggtaacca gctgttcgtt accgttgttg acaccacccg ttctaccaac 1980

atgaccctgt gcaccgaagt taccaaagaa ggtacctaca aaaacgacaa cttcaaagaa 2040

tacgttcgtc acgttgaaga atacgacctg cagttcgttt tccagctgtg caaaatcacc 2100

ctgaccgctg aaatcatgac ctacatccac accatggact ctaacatcct ggaagactgg 2160

cagttcggtc tgaccccgcc gccgtctgct tctctgcagg acacctaccg tttcgttacc 2220

tctcaggcta tcacctgcca gaaaaccgct ccgccgaaag aaaaagaaga cccgctgaac 2280

aaatacacct tctgggaagt taacctgaaa gaaaaattct ctgctgacct ggaccagttc 2340

ccgctgggtc gtaaattcct gctgcagtct ggtctgaaag ctaaaccgcg tctgaaacgt 2400

tctgctccga ccacccgtgc tccgtctacc aaacgtaaaa aagttaaaaa a2451

<210>13

<211>2622

<212>DNA

<213>人工序列

<400>13

atgaaaatcg aagaaggtaa actggtaatc tggattaacg gcgataaagg ctataacggt 60

ctcgctgaag tcggtaagaa attcgagaaa gataccggaa ttaaagtcac cgttgagcat120

ccggataaac tggaagagaa attcccacag gttgcggcaa ctggcgatgg ccctgacatt180

atcttctggg cacacgaccg ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc240

accccggaca aagcgttcca ggacaagctg tatccgttta cctgggatgc cgtacgttac300

aacggcaagc tgattgctta cccgatcgct gttgaagcgt tatcgctgat ttataacaaa360

gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga taaagaactg420

aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg480

ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgacattaaa540

gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt600

aaaaacaaac acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa660

ggcgaaacag cgatgaccat caacggcccg tgggcatggt ccaacatcga caccagcaaa720

gtgaattatg gtgtaacggt actgccgacc ttcaagggtc aaccatccaa accgttcgtt780

ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca aagagctggc aaaagagttc840

ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg900

ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga aagatccacg tattgccgcc960

actatggaaa acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc 1020

tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa 1080

gccctgaaag acgcgcagac taattcgagc tcgaacaaca acaacaataa caataacaac 1140

aacctcggga tcgagggaag gatttcagaa ttcatgtctg tttggcgtcc gtctgaagct 1200

accgtttacc tgccgccggt tccggtttct aaagttgttt ctaccgacga atacgtttct 1260

cgtacctcta tctactacta cgctggttct tctcgtctgc tggctgttgg taacccgtac 1320

ttctctatca aatctccgaa caacaacaaa aaagttctgg ttccgaaagt ttctggtctg 1380

cagtaccgtg ttttccgtgt tcgtctgccg gacccgaaca aattcggttt cccggacacc 1440

tctttctaca acccggacac ccagcgtctg gtttgggctt gcgttggtct ggaaatcggt 1500

cgtggtcagc cgctgggtgt tggtgtttct ggtcacccgt acttcaacaa attcgacgac 1560

accgaaacct ctaaccgtta cccggctcag ccgggttctg acaaccgtga atgcctgtct 1620

atggactaca aacagaccca gctgtgcctg atcggttgca aaccgccgac cggtgaacac 1680

tggggtaaag gtgttgcttg caacaacaac gctgctgcta ccgactgccc gccgctggaa 1740

ctgttcaact ctatcatcga agacggtgac atggttgaca ccggtttcgg ttgcatggac 1800

ttcggtaccc tgcaggctaa caaatctgac gttccgatcg acatctgcaa ctctacctgc 1860

aaatacccgg actacctgaa aatggcttct gaaccgtacg gtgactctct gttcttcttc 1920

ctgcgtcgtg aacagatgtt cgttcgtcac ttcttcaacc gtgctggtaa actgggtgaa 1980

gctgttccgg acgacctgta catcaaaggt tctggtaaca ccgctgttat ccagtcttct 2040

gctttcttcc cgaccccgtc tggttctatg gttacctctg aatctcagct gttcaacaaa 2100

ccgtactggc tgcagcgtgc tcagggtcac aacaacggta tctgctgggg taaccagctg 2160

ttcgttaccg ttgttgacac cacccgttct accaacatga ccctgtgcac cgaagttacc 2220

aaagaaggta cctacaaaaa cgacaacttc aaagaatacg ttcgtcacgt tgaagaatac 2280

gacctgcagt tcgttttcca gctgtgcaaa atcaccctga ccgctgaaat catgacctac 2340

atccacacca tggactctaa catcctggaa gactggcagt tcggtctgac cccgccgccg 2400

tctgcttctc tgcaggacac ctaccgtttc gttacctctc aggctatcac ctgccagaaa 2460

accgctccgc cgaaagaaaa agaagacccg ctgaacaaat acaccttctg ggaagttaac 2520

ctgaaagaaa aattctctgc tgacctggac cagttcccgc tgggtcgtaa attcctgctg 2580

cagtctggtc tgaaagctaa accgcgtctg aaacgttctg ct2622

<210>14

<211>2643

<212>DNA

<213>人工序列

<400>14

atgaaaatcg aagaaggtaa actggtaatc tggattaacg gcgataaagg ctataacggt 60

ctcgctgaag tcggtaagaa attcgagaaa gataccggaa ttaaagtcac cgttgagcat120

ccggataaac tggaagagaa attcccacag gttgcggcaa ctggcgatgg ccctgacatt180

atcttctggg cacacgaccg ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc240

accccggaca aagcgttcca ggacaagctg tatccgttta cctgggatgc cgtacgttac300

aacggcaagc tgattgctta cccgatcgct gttgaagcgt tatcgctgat ttataacaaa360

gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga taaagaactg420

aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg480

ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgacattaaa540

gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt600

aaaaacaaac acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa660

ggcgaaacag cgatgaccat caacggcccg tgggcatggt ccaacatcga caccagcaaa720

gtgaattatg gtgtaacggt actgccgacc ttcaagggtc aaccatccaa accgttcgtt780

ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca aagagctggc aaaagagttc840

ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg900

ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga aagatccacg tattgccgcc960

actatggaaa acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc 1020

tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa 1080

gccctgaaag acgcgcagac taattcgagc tcgaacaaca acaacaataa caataacaac 1140

aacctcggga tcgagggaag gatttcagaa ttcatgtctc tgtggctgcc gtctgaagct 1200

accgtttacc tgccgccggt tccggtttct aaagttgttt ctaccgacga atacgttgct 1260

cgtaccaaca tctactacca cgctggtacc tctcgtctgc tggctgttgg tcacccgtac 1320

ttcccgatca aaaaaccgaa caacaacaaa atcctggttc cgaaagtttc tggtctgcag 1380

taccgtgttt tccgtatcca cctgccggac ccgaacaaat tcggtttccc ggacacctct 1440

ttctacaacc cggacaccca gcgtctggtt tgggcttgcg ttggtgttga agttggtcgt 1500

ggtcagccgc tgggtgttgg tatctctggt cacccgctgc tgaacaaact ggacgacacc 1560

gaaaacgctt ctgcttacgc tgctaacgct ggtgttgaca accgtgaatg catctctatg 1620

gactacaaac agacccagct gtgcctgatc ggttgcaaac cgccgatcgg tgaacactgg 1680

ggtaaaggtt ctccgtgcac caacgttgct gttaacccgg gtgactgccc gccgctggaa 1740

ctgatcaaca ccgttatcca ggacggtgac atggttgaca ccggtttcgg tgctatggac 1800

ttcaccaccc tgcaggctaa caaatctgaa gttccgctgg acatctgcac ctctatctgc 1860

aaatacccgg actacatcaa aatggtttct gaaccgtacg gtgactctct gttcttctac 1920

ctgcgtcgtg aacagatgtt cgttcgtcac ctgttcaacc gtgctggtgc tgttggtgaa 1980

aacgttccgg acgacctgta catcaaaggt tctggttcta ccgctaacct ggcttcttct 2040

aactacttcc cgaccccgtc tggttctatg gttacctctg acgctcagat cttcaacaaa 2100

ccgtactggc tgcagcgtgc tcagggtcac aacaacggta tctgctgggg taaccagctg 2160

ttcgttaccg ttgttgacac cacccgttct accaacatgt ctctgtgcgc tgctatctct 2220

acctctgaaa ccacctacaa aaacaccaac ttcaaagaat acctgcgtca cggtgaagaa 2280

tacgacctgc agttcatctt ccagctgtgc aaaatcaccc tgaccgctga cgttatgacc 2340

tacatccact ctatgaactc taccatcctg gaagactgga acttcggtct gcagccgccg 2400

ccgggtggta ccctggaaga cacctaccgt ttcgttacct ctcaggctat cgcttgccag 2460

aaacacaccc cgccggctcc gaaagaagac ccgctgaaaa aatacacctt ctgggaagtt 2520

aacctgaaag aaaaattctc tgctgacctg gaccagttcc cgctgggtcg taaattcctg 2580

ctgcaggctg gtctgaaagc taaaccgaaa ttcaccctgg gtaaacgtaa agctaccccg 2640

acc 2643

<210>15

<211>2649

<212>DNA

<213>人工序列

<400>15

atgaaaatcg aagaaggtaa actggtaatc tggattaacg gcgataaagg ctataacggt 60

ctcgctgaag tcggtaagaa attcgagaaa gataccggaa ttaaagtcac cgttgagcat120

ccggataaac tggaagagaa attcccacag gttgcggcaa ctggcgatgg ccctgacatt180

atcttctggg cacacgaccg ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc240

accccggaca aagcgttcca ggacaagctg tatccgttta cctgggatgc cgtacgttac300

aacggcaagc tgattgctta cccgatcgct gttgaagcgt tatcgctgat ttataacaaa360

gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga taaagaactg420

aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg480

ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgacattaaa540

gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt600

aaaaacaaac acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa660

ggcgaaacag cgatgaccat caacggcccg tgggcatggt ccaacatcga caccagcaaa720

gtgaattatg gtgtaacggt actgccgacc ttcaagggtc aaccatccaa accgttcgtt780

ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca aagagctggc aaaagagttc840

ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg900

ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga aagatccacg tattgccgcc960

actatggaaa acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc 1020

tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa 1080

gccctgaaag acgcgcagac taattcgagc tcgaacaaca acaacaataa caataacaac 1140

aacctcggga tcgagggaag gatttcagaa ttcatggctc tgtggcgtcc gtctgacaac 1200

accgtttacc tgccgccgcc gtctgttgct cgtgttgtta acaccgacga ctacgttacc 1260

cgtacctcta tcttctacca cgctggttct tctcgtctgc tgaccgttgg taacccgtac 1320

ttccgtgttc cggctggtgg tggtaacaaa caggacatcc cgaaagtttc tgcttaccag 1380

taccgtgttt tccgtgttca gctgccggac ccgaacaaat tcggtctgcc ggacaactct 1440

atctacaacc cggaaaccca gcgtctggtt tgggcttgcg ctggtgttga aatcggtcgt 1500

ggtcagccgc tgggtgttgg tctgtctggt cacccgttct acaacaaact ggacgacacc 1560

gaatcttctc acgctgctac ctctaacgtt tctgaagacg ttcgtgacaa cgtttctgtt 1620

gactacaaac agacccagct gtgcatcctg ggttgcgctc cggctatcgg tgaacactgg 1680

gctaaaggta ccgcttgcaa atctcgtccg ctgtctcagg gtgactgccc gccgctggaa 1740

ctgaaaaaca ccgttctgga agacggtgac atggttgaca ccggttacgg tgctatggac 1800

ttctctaccc tgcaggacac caaatgcgaa gttccgctgg acatctgcca gtctatctgc 1860

aaatacccgg actacctgca gatgtctgct gacccgtacg gtgactctat gttcttctgc 1920

ctgcgtcgtg aacagctgtt cgctcgtcac ttctggaacc gtgctggtac catgggtgac 1980

accgttccgc agtctctgta catcaaaggt accggtatgc gtgcttctcc gggttcttgc 2040

gtttactctc cgtctccgtc tggttctatc gttacctctg actctcagct gttcaacaaa 2100

ccgtactggc tgcacaaagc tcagggtcac aacaacggtg tttgctggca caaccagctg 2160

ttcgttaccg ttgttgacac cacccgttct accaacctga ccatctgcgc ttctacccag 2220

tctccggttc cgggtcagta cgacgctacc aaattcaaac agtactctcg tcacgttgaa 2280

gaatacgacc tgcagttcat cttccagctg tgcaccatca ccctgaccgc tgacgttatg 2340

tcttacatcc actctatgaa ctcttctatc ctggaagact ggaacttcgg tgttccgccg 2400

ccgccgacca cctctctggt tgacacctac cgtttcgttc agtctgttgc tatcacctgc 2460

cagaaagacg ctgctccggc tgaaaacaaa gacccgtacg acaaactgaa attctggaac 2520

gttgacctga aagaaaaatt ctctctggac ctggaccagt acccgctggg tcgtaaattc 2580

ctggttcagg ctggtctgcg tcgtaaaccg accatcggtc cgcgtaaacg ttctgctccg 2640

tctgctacc 2649