CellTiter Glo Luminescent Cell Viability Assay Protocol

Technical Bulletin

CellTiter-Glo

®

Luminescent

Cell Viability Assay

INSTRUCTIONS FOR USE OF PRODUCTS G7570, G7571, G7572 AND

G7573.

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INSTRUMENTS

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PRINTED IN USA.

Revised 12/12Part# TB288

CellTiter-Glo

®

Luminescent

Cell Viability Assay

All technical literature is available on the Internet at: /protocols/

Please visit the web site to verify that you are using the most current version of this

Technical Bulletin. Please contact Promega Technical Services if you have questions on use

ofthissystem.E-mail:********************

1

t Components and .4

ming the CellTiter-Glo

®

5

.5

ol for the Cell .6

ol for Generating an ATP Standard Curve (optional).........................7

ix.............................................................................................................7

A.

B.

C.

D.

Overview of the CellTiter-Glo

®

7

8

11

12

ption

The CellTiter-Glo

®

Luminescent Cell Viability Assay

(a–e)

is a homogeneous

method to determine the number of viable cells in culture based on quantitation

of the ATP present, which signals the presence of metabolically active cells. The

CellTiter-Glo

®

Assay is designed for use with multiwell-plate formats, making

it ideal for automated high-throughput screening (HTS) and cell proliferation

and cytotoxicity assays. The homogeneous assay procedure (Figure 1) involves

adding a single reagent (CellTiter-Glo

®

Reagent) directly to cells cultured in

serum-supplemented medium. Cell washing, removal of medium or multiple

pipetting steps are not required.

The homogeneous “add-mix-measure” format results in cell lysis and generation

of a luminescent signal proportional to the amount of ATP present (Figure 2).

The amount of ATP is directly proportional to the number of cells present in

culture in agreement with previous reports (1). The CellTiter-Glo

®

Assay relies on

the properties of a proprietary thermostable luciferase (Ultra-Glo™ Recombinant

Luciferase), which generates a stable “glow-type” luminescent signal and

improves performance across a wide range of assay conditions. The luciferase

reaction for this assay is shown in Figure 3. The half-life of the luminescent signal

resulting from this reaction is greater than five hours (Figure4). This extended

half-life eliminates the need for reagent injectors and provides flexibility for

continuous or batch-mode processing of multiple plates. The unique

homogeneous format reduces pipetting errors that may be introduced during

the multiple steps required by other ATP-measurement methods.

Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA

Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·

Printed in USA.

Revised 12/12

Part# TB288

Page 1

CellTiter-Glo

®

Substrate

CellTiter-Glo

®

Buffer

CellTiter-Glo

®

Reagent

Mixer

Luminometer

Figure 1. Flow diagram showing preparation and use of CellTiter-Glo

®

Reagent.

Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA

Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·

Part# TB288

Page 2

Printed in USA.

Revised 12/12

3

1

7

0

M

A

1

2

_

0

A

System Advantages

•

•

•

•

•

•

Homogeneous:“Add-mix-measure” format reduces the number of plate-

handling steps to fewer than that required for similar ATP assays.

Fast:Data can be recorded 10 minutes after adding reagent.

Sensitive:Measures cells at numbers below the detection limits of

standard colorimetric and fluorometric assays.

Flexible:Can be used with various multiwell formats. Data can be

recorded by luminometer or CCD camera or imaging device.

Robust:Luminescent signal is very stable, with a half-life >5 hours,

depending on cell type and culture medium used.

Able to Multiplex:Can be used with reporter gene assays or other

cell-based assays from Promega (2,3).

4.0 × 10

6

R² = 0.999

L

u

m

i

n

e

s

c

e

n

c

e

(

R

L

U

)

3.5 × 10

6

3.0 × 10

6

2.5 × 10

6

2.0 × 10

6

1.5 × 10

6

1.0 × 10

6

0.5 × 10

6

r² = 0.99

50,000

40,000

30,000

20,000

10,000

0

0

r² = 0.99

0

10,000

20,00030,00040,00050,000

60,000

Cells per Well

Figure 2. Cell number correlates with luminescent output.A direct relationship

exists between luminescence measured with the CellTiter-Glo

®

Assay and the number

of cells in culture over three orders of magnitude. Serial twofold dilutions of HEK293

cells were made in a 96-well plate in DMEM with 10% FBS, and assays were

performed as described in Section3.B. Luminescence was recorded 10minutes after

reagent addition using a GloMax

®

-Multi+ Detection System. Values represent the

mean ± S.D. of four replicates for each cell number. The luminescent signal from

50HEK293 cells is greater than three times the background signal from serum-

supplemented medium without cells. There is a linear relationship (r

2

= 0.99)

between the luminescent signal and the number of cells from 0to 50,000 cells per well.

HO

S

N

COOH

+ATP+O

2

N

S

Ultra-Glo™ Recombinant

Luciferase

Mg

2+

O

S

N

N

S

O

0

+AMP+PP

i

+CO

2

+Light

Beetle LuciferinOxyluciferin

Figure 3. The luciferase -oxygenation of luciferin is catalyzed by

luciferase in the presence of Mg

2+

, ATP and molecular oxygen.

Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA

Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·

Printed in USA.

Revised 12/12

Part# TB288

Page 3

3

1

7

1

M

A

1

2

_

0

A

0

R

e

l

a

t

i

v

e

L

u

m

i

n

e

s

c

e

n

c

e

(

%

)

250300

Time (minutes)

Figure 4. Extended luminescent half-life allows high-throughput batch

stability is shown for three common cell lines. HepG2 and BHK-21

cells were grown and assayed in MEM containing 10% FBS, while CHO-K1 cells

were grown and assayed in DME/F-12 containing 10% FBS. CHO-K1, BHK-21 and

HepG2 cells, at 25,000 cells per well, were added to a 96-well plate. After an equal

volume of CellTiter-Glo

®

Reagent was added, plates were shaken and luminescence

monitored over time with the plates held at 22°C. The half-lives of the luminescent

signals for the CHO-K1, BHK-21 and HepG2 cells were approximately 5.4, 5.2 and

5.8hours, respectively.

t Components and Storage Conditions

ProductSizeCat.#

CellTiter-Glo

®

Luminescent Cell Viability Assay10mlG7570

Substrate is sufficient for 100 assays at 100µl/assay in 96-well plates or 400 assays at

25µl/assay in 384-well plates. Includes:

•1 × 10ml

•1 vial

CellTiter-Glo

®

Buffer

CellTiter-Glo

®

Substrate (lyophilized)

ProductSizeCat.#

CellTiter-Glo

®

Luminescent Cell Viability Assay10 × 10mlG7571

Each vial of substrate is sufficient for 100 assays at 100µl/assay in 96-well plates or

400 assays at 25µl/assay in 384-well plates (1,000 to 4,000 total assays). Includes:

•10 × 10ml

•10 vials

CellTiter-Glo

®

Buffer

CellTiter-Glo

®

Substrate (lyophilized)

Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA

Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·

Part# TB288

Page 4

Printed in USA.

Revised 12/12

3

1

7

3

M

A

1

2

_

0

A

100

90

80

70

60

50

40

30

20

10

0

CHO-K1

BHK-21

HepG2

ProductSizeCat.#

CellTiter-Glo

®

Luminescent Cell Viability Assay100mlG7572

Substrate is sufficient for 1,000 assays at 100µl/assay in 96-well plates or 4,000

assays at 25µl/assay in 384-well plates. Includes:

•1 × 100ml

•1 vial

CellTiter-Glo

®

Buffer

CellTiter-Glo

®

Substrate (lyophilized)

ProductSizeCat.#

CellTiter-Glo

®

Luminescent Cell Viability Assay10 × 100mlG7573

Each vial of substrate is sufficient for 1,000 assays at 100µl/assay in 96-well plates or

4,000 assays at 25µl/assay in 384-well plates (10,000to 40,000 total assays). Includes:

•10 × 100ml

•10 vials

CellTiter-Glo

®

Buffer

CellTiter-Glo

®

Substrate (lyophilized)

Storage Conditions:For long-term storage, store the lyophilized CellTiter-Glo

®

Substrate and CellTiter-Glo

®

Buffer at –20°C. For frequent use, the CellTiter-Glo

®

Buffer can be stored at 4°C or room temperature for 48hours without loss of

activity. See product label for expiration date information. Reconstituted

CellTiter-Glo

®

Reagent (Buffer plus Substrate) can be stored at room temperature

for up to 8hours with <10% loss of activity, at 4°C for 48hours with ~5% loss

of activity, at 4°C for 4days with ~20% loss of activity or at –20°C for 21weeks

with ~3% loss of activity. The reagent is stable for up to ten freeze-thaw cycles,

with less than 10% loss of activity.

ming the CellTiter-Glo

®

Assay

Materials to Be Supplied by the User

•opaque-walled multiwell plates adequate for cell culture

•multichannel pipette or automated pipetting station for reagent delivery

•device (plate shaker) for mixing multiwell plates

•luminometer, CCD camera or imaging device capable of reading multiwell plates

•optional:ATP for use in generating a standard curve (Section 3.C)

t Preparation

the CellTiter-Glo

®

Buffer, and equilibrate to room temperature prior

to use. For convenience the CellTiter-Glo

®

Buffer may be thawed and

stored at room temperature for up to 48hours prior to use.

brate the lyophilized CellTiter-Glo

®

Substrate to room temperature

prior to use.

Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA

Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·

Printed in USA.

Revised 12/12

Part# TB288

Page 5

t Preparation (continued)

er the appropriate volume (10ml for Cat.# G7570 and G7571, or 100ml

for Cat.# G7572 and G7573) of CellTiter-Glo

®

Buffer into the amber bottle

containing CellTiter-Glo

®

Substrate to reconstitute the lyophilized

enzyme/substrate mixture. This forms the CellTiter-Glo

®

Reagent.

by gently vortexing, swirling or inverting the contents to obtain a

homogeneous solution. The CellTiter-Glo

®

Substrate should go into

solution easily in less than 1minute.

ol for the Cell Viability Assay

We recommend that you perform a titration of your particular cells to

determine the optimal number and ensure that you are working within the

linear range of the CellTiter-Glo

®

Assay. Figure2 provides an example of such

a titration of HEK293 cells using 0 to 50,000 cells per well in a 96-well format.

e opaque-walled multiwell plates with mammalian cells in culture

medium, 100µl per well for 96-well plates or 25µl per well for 384-well

plates.

!

Multiwell plates must be compatible with the luminometer used.

e control wells containing medium without cells to obtain a value for

background luminescence.

the test compound to experimental wells, and incubate according to

culture protocol.

brate the plate and its contents at room temperature for

approximately 30 minutes.

a volume of CellTiter-Glo

®

Reagent equal to the volume of cell culture

medium present in each well (e.g., add 100µl of reagent to 100µl of medium

containing cells for a 96-well plate, or add 25µl of reagent to 25µl of

medium containing cells for a 384-well plate).

contents for 2 minutes on an orbital shaker to induce cell lysis.

the plate to incubate at room temperature for 10 minutes to stabilize

luminescent signal.

Note:Uneven luminescent signal within standard plates can be caused by

temperature gradients, uneven seeding of cells or edge effects in multiwell

plates.

luminescence.

Note:Instrument settings depend on the manufacturer. An integration time

of 0.25–1 second per well should serve as a guideline.

Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA

Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·

Part# TB288

Page 6

Printed in USA.

Revised 12/12

ol for Generating an ATP Standard Curve (optional)

It is a good practice to generate a standard curve using the same plate on

which samples are assayed. We recommend ATP disodium salt (Cat.# P1132,

Sigma Cat.# A7699 or GE Healthcare Cat.# 27-1006). The ATP standard curve

should be generated immediately prior to adding the CellTiter-Glo

®

Reagent

because endogenous ATPase enzymes found in sera may reduce ATP levels.

e 1µM ATP in culture medium (100µl of 1µM ATP solution contains

10

–10

moles ATP).

e serial tenfold dilutions of ATP in culture medium (1µM to 10nM;

100µl contains 10

–10

to 10

–12

moles of ATP).

e a multiwell plate with varying concentrations of ATP standard in

100µl medium (25µl for a 384-well plate).

a volume of CellTiter-Glo

®

Reagent equal to the volume of ATP

standard present in each well.

contents for 2 minutes on an orbital shaker.

the plate to incubate at room temperature for 10 minutes to stabilize

the luminescent signal.

luminescence.

ix

ew of the CellTiter-Glo

®

Assay

The assay system uses the properties of a proprietary thermostable luciferase to

enable reaction conditions that generate a stable “glow-type” luminescent

signal while simultaneously inhibiting endogenous enzymes released during

cell lysis (e.g., ATPases). Release of ATPases will interfere with accurate ATP

measurement. Historically, firefly luciferase purified from Photinus pyralis

(LucPpy) has been used in reagents for ATP assays (1,4–7). However, it has

only moderate stability in vitro and is sensitive to its chemical environment,

including factors such as pH and detergents, limiting its usefulness for

developing a robust homogeneous ATP assay. Promega has successfully

developed a stable form of luciferase based on the gene from another firefly,

Photuris pennsylvanica(LucPpe2), using an approach to select characteristics that

improve performance in ATP assays. The unique characteristics of this mutant

(LucPpe2

m

) enabled design of a homogeneous single-reagent-addition approach

to perform ATP assays with cultured cells. Properties of the CellTiter-Glo

®

Reagent overcome the problems caused by factors, such as ATPases, that

interfere with ATP measurement in cell extracts. The reagent is physically

robust and provides a sensitive and stable luminescent output.

Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA

Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·

Printed in USA.

Revised 12/12

Part# TB288

Page 7

ew of the CellTiter-Glo

®

Assay (continued)

Sensitivity and Linearity:The ATP-based detection of cells is more sensitive

than other methods (8–10). In experiments performed by Promega scientists,

the luminescent signal from 50HEK293 cells is greater than three standard

deviations above the background signal from serum-supplemented medium

without cells. There is a linear relationship (r

2

= 0.99) between the luminescent

signal and the number of cells from 0 to 50,000 cells per well in the 96-well

format. The luminescence values in Figure 2 were recorded after 10minutes of

incubation at room temperature to stabilize the luminescent signal as described

in Section3.B. Incubation of the same 96-well plate used in the experiment

shown in Figure 2 for 360minutes at room temperature had little effect on the

relationship between luminescent signal and number of cells (r

2

= 0.99).

Speed:The homogeneous procedure to measure ATP using the CellTiter-Glo

®

Assay is quicker than other ATP assay methods that require multiple steps to

extract ATP and measure luminescence. The CellTiter-Glo

®

Assay also is faster

than other commonly used methods to measure the number of viable cells

(such as MTT, alamarBlue

®

or Calcein-AM) that require prolonged incubation

steps to enable the cells’ metabolic machinery to convert indicator molecules

into a detectable signal.

onal Considerations

Temperature:The intensity and decay rate of the luminescent signal from the

CellTiter-Glo

®

Assay depends on the luciferase reaction rate. Environmental

factors that affect the luciferase reaction rate will change the intensity and

stability of the luminescent signal. Temperature is one factor that affects the

rate of this enzymatic assay and thus the light output. For consistent results,

equilibrate assay plates to a constant temperature before performing the assay.

Transferring eukaryotic cells from 37°C to room temperature has little effect on

ATP content (5). We have demonstrated that removing cultured cells from a

37°C incubator and allowing them to equilibrate to 22°C for 1–2 hours had

little effect on ATP content. For batch-mode processing of multiple assay

plates, take precautions to ensure complete temperature equilibration. Plates

removed from a 37°C incubator and placed in tall stacks at room temperature

will require longer equilibration than plates arranged in a single layer.

Insufficient equilibration may result in a temperature gradient effect between

wells in the center and at the edge of the plates. The temperature gradient

pattern also may depend on the position of the plate in the stack.

Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA

Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·

Part# TB288

Page 8

Printed in USA.

Revised 12/12

Chemicals:The chemical environment of the luciferase reaction affects the

enzymatic rate and thus luminescence intensity. Differences in luminescence

intensity have been observed using different types of culture media and sera.

The presence of phenol red in culture medium should have little impact on

luminescence output. Assaying 0.1µM ATP in RPMI medium without phenol

red resulted in ~5% increase in luminescence output (in relative light units

[RLU]) compared to assays in RPMI containing the standard concentration of

phenol red, whereas assays in RPMI medium containing twice the normal

concentration of phenol red showed a ~2% decrease in luminescence.

Solvents for the various test compounds may interfere with the luciferase

reaction and thus the light output from the assay. Interference with the

luciferase reaction can be detected by assaying a parallel set of control wells

containing medium without cells. Dimethylsulfoxide (DMSO), commonly used

as a vehicle to solubilize organic chemicals, has been tested at final

concentrations of up to 2% in the assay and only minimally affects light output.

Plate Recommendations:We recommend using standard opaque-walled

multiwell plates suitable for luminescence measurements. Opaque-walled

plates with clear bottoms to allow microscopic visualization of cells also may

be used; however, these plates will have diminished signal intensity and

greater cross talk between wells. Opaque white tape may be used to decrease

luminescence loss and cross talk.

Cellular ATP Content:Different cell types have different amounts of ATP,

and values reported for the ATP level in cells vary considerably (1,4,11–13).

Factors that affect the ATP content of cells may affect the relationship between

cell number and luminescence. Anchorage-dependent cells that undergo

contact inhibition at high densities may show a change in ATP content per cell

at high densities, resulting in a nonlinear relationship between cell number

and luminescence. Factors that affect the cytoplasmic volume or physiology of

cells also will affect ATP content. For example, oxygen depletion is one factor

known to cause a rapid decrease in ATP (1).

Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA

Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·

Printed in USA.

Revised 12/12

Part# TB288

Page 9

onal Considerations (continued)

Mixing:Optimal assay performance is achieved when the CellTiter-Glo

®

Reagent is mixed completely with the cultured cells. Suspension cell lines (e.g.,

Jurkat cells) generally require less mixing to achieve lysis and extract ATP than

adherent cells (e.g., L929 cells). Tests were done to evaluate the effect of

shaking the plate after adding the CellTiter-Glo

®

Reagent. Suspension cells

cultured in multiwell plates showed only minor differences in light output

whether or not the plates were shaken after adding the CellTiter-Glo

®

Reagent.

Adherent cells are more difficult to lyse and show a substantial difference

between shaken and nonshaken plates.

Several additional parameters related to reagent mixing include the force of

delivery of CellTiter-Glo

®

Reagent, sample volume and dimensions of the well.

All of these factors may affect assay performance. The degree of reagent mixing

required may be affected by the method used to add the CellTiter-Glo

®

Reagent

to the assay plates. Automated pipetting devices using a greater or lesser force

of fluid delivery may affect the degree of subsequent mixing required.

Complete reagent mixing in 96-well plates should be achieved using orbital

plate shaking devices built into many luminometers and the recommended

2-minute shaking time. Special electromagnetic shaking devices that use a

radius smaller than the well diameter may be required to efficiently mix

contents of 384-well plates. The depth of medium and geometry of the

multiwell plates may have an effect on mixing efficiency. We recommend that

you take these factors into consideration when performing the assay and

empirically determine whether a mixing step is necessary for the individual

application.

Luminometers

For highly sensitive luminometric assays, the luminometer model and settings

greatly affect the quality of data obtained. Luminometers from different

manufacturers will vary in sensitivities and dynamic ranges. We recommend

the GloMax

®

products because these instruments do not require gain

adjustments to achieve optimal sensitivity and dynamic range. Additionally,

GloMax

®

instruments are preloaded with Promega protocols for ease of use.

If you are not using a GloMax

®

luminometer, consult the operating manual for

your luminometer to determine the optimal settings. The limits should be

verified on each instrument before analysis of experimental samples. The assay

should be linear in some portion of the detection range of the instrument used.

For an individual luminometer there may be different gain settings. We

recommend that you optimize the gain settings.

Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA

Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·

Part# TB288

Page 10

Printed in USA.

Revised 12/12

nces

, S.P. et al.(1993) The use of ATP bioluminescence as a measure of cell

proliferation and cytotoxicity. J. Immunol. Methods160, 81–8.

, al.(2004) Multiplexing homogeneous cell-based assays. Cell Notes10, 2–5.

, T., Moravec, R. and Niles, A. (2005) Selecting cell-based assays for drug

discovery screening. Cell Notes13, 16–21.

, L., Grönroos, M. and Nieminen, A.L. (1984) Bioluminescence of cellular ATP:

A new method for evaluating cytotoxic agents in vitro. Med. Biol.62, 338–43.

, A. et al.(1986) Estimation of biomass in growing cell lines by adenosine

triphosphate s Enzymol. 133, 27–42.

, B.U. et al.(1988) Application of an ATP-bioluminescence assay in human tumor

chemosensitivity testing. Gynecol. Oncol.31, 191–204.

dt, al.(1991) Characterization of in vitro chemosensitivity of

perioperative human ovarian malignancies by adenosine triphosphate

chemosensitivity assay. Am. J. Obstet. Gynecol. 165, 245–55.

, R.D. et al.(1995) Comparison of MTT and ATP-based assays for the

measurement of viable cell number. J. Biolumin. Chemilumin.10, 29–34.

, I.A. et al.(1995) Methotrexate chemosensitivity by ATP luminescence in human

leukemia cell lines and in breast cancer primary cultures: Comparison of the TCA-100

assay with a clonogenic assay. AntiCancer Drugs6, 398–404.

a, Y. et al.(1987) The ATP assay is more sensitive than the succinate

dehydrogenase inhibition test for predicting cell viability. Eur. J. Cancer Clin. Oncol.23,

273–6.

y, P.E. (1986) Extraction of adenosine triphosphate from microbial and somatic

cells. Methods Enzymol.133, 14–22.

s, B. et al.(1986) Application of intracellular ATP determination in lymphocytes

for HLA-typing. J. Biolumin. Chemilumin.1, 47–51.

tti, P.E. et al.(1995) Chemosensitivity testing of human tumors using a

microplate adenosine triphosphate luminescence assay: Clinical correlation for

cisplatin resistance of ovarian carcinoma. Cancer Res. 55, 5276–82.

Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA

Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·

Printed in USA.

Revised 12/12

Part# TB288

Page 11

d Products

Cell Proliferation Products

Product

ApoLive-Glo™ Multiplex Assay

ApoTox-Glo™ Triplex Assay

CellTiter-Fluor™ Cell Viability Assay (fluorescent)

CellTiter-Blue

®

Cell Viability Assay (resazurin)

CellTiter 96

®

AQ

ueous

One Solution

Cell Proliferation Assay (MTS, colorimetric)

CellTiter 96

®

AQ

ueous

Non-Radioactive

Cell Proliferation Assay (MTS, colorimetric)

CellTiter 96

®

AQ

ueous

MTS Reagent Powder

CellTiter 96

®

Non-Radioactive

Cell Proliferation Assay (MTT, colorimetric)

Additional sizes available.

Size

10ml

10ml

10ml

20ml

200 assays

1,000 assays

1g

1,000 assays

Cat.#

G6410

G6320

G6080

G8080

G3582

G5421

G1111

G4000

Cytotoxicity Assays

Product

CytoTox-Glo™ Cytotoxicity Assay (luminescent)*

Mitochondrial ToxGlo™ Assay*

MultiTox-Glo Multiplex Cytotoxicity Assay

(luminescent, fluorescent)*

MultiTox-Fluor Multiplex Cytotoxicity Assay

(fluorescent)*

CytoTox-Fluor™ Cytotoxicity Assay (fluorescent)*

CytoTox-ONE™ Homogeneous Membrane

Integrity Assay (LDH, fluorometric)*

CytoTox-ONE™ Homogeneous Membrane

Integrity Assay, HTP

CytoTox 96

®

Non-Radioactive Cytotoxicity Assay

(LDH, colorimetric)*

GSH-Glo™ Glutathione Assay

GSH/GSSG-Glo™ Assay

*Additional sizes available.

Size

10ml

10ml

10ml

10ml

10ml

200–800 assays

1,000–4,000 assays

1,000 assays

10ml

50ml

10ml

50ml

Cat.#

G9290

G8000

G9270

G9200

G9260

G7890

G7892

G1780

V6911

V6912

V6611

V6612

Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA

Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·

Part# TB288

Page 12

Printed in USA.

Revised 12/12

Luminometers

Product

GloMax

®

-Multi+ Detection System with Instinct™ Software:

Base Instrument with Shaking

GloMax

®

-Multi+ Detection System with Instinct™ Software:

Base Instrument with Heating and Shaking

GloMax

®

-Multi+ Luminescence Module

Apoptosis Products

Product Size

Caspase-Glo

®

2 Assay*10ml

Caspase-Glo

®

6 Assay*10ml

Caspase-Glo

®

3/7 Assay*2.5ml

Caspase-Glo

®

8 Assay*2.5ml

Caspase-Glo

®

9 Assay*2.5ml

Apo-ONE

®

Homogeneous Caspase-3/7 Assay1ml

DeadEnd™ Fluorometric TUNEL System60 reactions

DeadEnd™ Colorimetric TUNEL System20 reactions

Anti-ACTIVE

®

Caspase-3 pAb50µl

Anti-PARP p85 Fragment pAb50µl

Anti-pS

473

Akt pAb40µl

Caspase Inhibitor Z-VAD-FMK, 20mM50µl

125µl

*Additional sizes available.

Size

1 each

1 each

1 each

Cat.#

E8032

E9032

E8041

Cat.#

G0940

G0970

G8090

G8200

G8210

G7792

G3250

G7360

G7481

G7341

G7441

G7231

G7232

(a)

U.S. Pat. Nos. 6,602,677 and 7,241,584, European Pat. No. 1131441, Japanese Pat. Nos. 4537573 and 4520084 and other patents

pending

(b)

U.S. Pat. No. 7,741,067, Japanese Pat. No. 4485470 and other patents pending.

(c)

U.S. Pat. No. 7,700,310, European Pat. No. 1546374 and other patents pending.

(d)

U.S. Pat. Nos 7,083,911, 7,452,663 and 7,732,128, European Pat. No. 1383914 and Japanese Pat. Nos. 4125600 and 4275715.

(e)

The method of recombinant expression of Coleopteraluciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

© 2001–2012 Promega Corporation. All Rights Reserved.

Anti-ACTIVE, Apo-ONE, Caspase-Glo, CellTiter 96, CellTiter-Blue, CellTiter-Glo, CytoTox 96 and GloMax are registered

trademarks of Promega Corporation. ApoTox-Glo, ApoLive-Glo, CellTiter-Fluor, CytoTox-Fluor, CytoTox-Glo, CytoTox-ONE,

DeadEnd, GSH-Glo, GSH/GSSG-Glo, Instinct, Mitochondrial ToxGlo and Ultra-Glo are trademarks of Promega Corporation.

alamarBlue is a registered trademark of Trek Diagnostic Ssystems, Inc.

Products may be covered by pending or issued patents or may have certain limitations. Please visit our Web site for more

information.

All prices and specifications are subject to change without prior notice.

Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the

most up-to-date information on Promega products.

Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA

Toll Free in USA 800-356-9526·Phone 608-274-4330 ·Fax 608-277-2516 ·

Printed in USA.

Revised 12/12

Part# TB288

Page 13